Fig. 3. TRPV4 directly affects proliferation and apoptosis in HEI-OC1 cells. (A) The effect of TRPV4 on cell proliferation of HEI-OC1 cells was detected using the MTT assay. (B) The effect of TRPV4 on cell apoptosis of HEI-OC1 cells was detected using the cell death detection ELISA-Plus apoptosis assay. Data are representative of three experiments. ^*indicates differences from euglycemic controls at p<0.05, ^†Indicates differences from euglycemic controls at p<0.01, ^‡Indicates differences from untreated controls at p<0.05, respectively. TRPV4, transient receptor potential vanilloid sub type 4; HC, TRPV4 antagonist HC-067047; GSK, TRPV4 agonist GSK1016790A.