Fig. 4. S100A4 KO reduces liver tissue damage and inflammatory cell infiltration in MCD diet-induced NAFLD mice. (A) HE staining, ORO staining, and Masson staining were used to observe pathological changes of liver tissues in each group (×400); (B–E) Comparison of steatosis (B), lobular inflammation (C), hepatocyte ballooning (D), total NAS (E), and fibrosis (F) in liver tissues of mice in each group. ^*p<0.05 compared to MCS group, ^†p<0.05 compared to WT/MCD group. S100A4, S100 calcium binding protein A4; MCD, methionine-choline-deficient; MCS, methionine-choline-sufficient; KO, knockout; WT, wild-type; HE, hematoxylin and eosin; ORO, Oil Red O; NAFLD, non-alcoholic fatty liver; NAS, NAFLD activity scores.