Fig. 2.

Activity of AN1602 on amorphous cellulose (PASC). a MALDI-ToF spectra of products generated from the incubation of PASC with AN1602. Avicel (1%, w/v) was incubated with AN1602 (1 µM) in 50 mM sodium acetate (pH 5.5) in the presence of 1 mM of ascorbic acid at 45 °C for 24 h. b HPAEC chromatogram showing the generation of native and oxidized oligosaccharides after incubation of PASC with AN1602. A mixture of non-oxidized cello-oligosaccharide (DP2-6) standard as control run for peak annotation is also shown. Analysis was carried out after treating PASC under similar conditions as described in a. nC nanocoulomb