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. 2018 Oct 17;8:171. doi: 10.1186/s13568-018-0701-5

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Activity of AN1602 on cellohexaose. a PACE gel showing digestion products from cellohexaose upon AN1602 activity in the presence of 1 mM ascorbic acid. Lane 1—cellohexaose, lane 2—cellohexaose treated with AN1602. The migration standards (M) are cello-oligosaccharides corresponding to Glc2 (cellobiose), Glc3 (cellotriose), Glc4 (cellotetraose) and Glc6 (cellohexaose). b HPAEC chromatogram showing the generation of native and oxidized oligosaccharides after incubating cellohexaose (0.5 mg/mL) with AN1602. A mixture of nonoxidized cello-oligosaccharide (DP2-6) standards in the control run is also shown. Analysis was carried out after treating cellohexaose with AN1602 under similar conditions as in Fig. 2a. nC nanocoulomb