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. 2018 Oct 17;8:15314. doi: 10.1038/s41598-018-33686-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tdc2-Gal4-positive arborizations in a whole fly section. Projection of one medial sagittal agarose section of 80 µm thickness labeled by anti-GFP to visualize membranes of Tdc2-Gal4-positive neurons (green in (A), black in (B)); Phalloidin, anti-Fasciclin2 (Fas2) and anti-Bruchpilot (Brp) to visualize muscles, cells and synapses, respectively (magenta in (A), black in (C)) and DAPI to mark cell bodies (blue in (A), black in (D)). (E) A single optical section showing the bright-field picture. Scale bars = 50 µm.