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. 2018 Oct 17;8:15314. doi: 10.1038/s41598-018-33686-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Tdc2-Gal4-positive innervation of the reproductive organs. Projections of optical sections visualizing the innervation pattern of Tdc2-Gal4-positive neurons (Tdc2Ns; green) of reproductive organs visualized by DAPI, Phalloidin and Fasciclin2 staining (magenta). (A,B) Tdc2N arborization pattern in a sagittal section of a male (A) and female (B) abdomen, respectively. (C–F) The anterior ejaculatory duct (ED’), muscles of the ejaculatory bulb (EB), the vas deferens (VD) and seminal vesicle (SV) are innervated by Tdc2Ns. (G–I) Tdc2Ns arborize onto the common and lateral oviduct (CO, LO), spermathecal duct (arrowhead in G), uterus (UT) muscles (arrowhead in H) and the ovaries (OV). (J) Schematic drawing of a male fly visualizing the ED’ (brown), EB muscles (dark brown) and the VD and SV (light brown) shown in A,C-F. (K) Schematic drawing of a female fly visualizing the UT (brown), spermatheca (SP, dark brown), CO and LO (light brown) and the OV (grey) shown in B,G-I. SR, seminal receptacle; TE, testes. Scale bars = 50 µm.