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. 2018 Oct 17;4:49. doi: 10.1038/s41420-018-0113-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Fluorescence staining analysis showed that AQP2, ERα, and ERβ were mainly located in the outer part of the nucleus (a). Target protein was stained in red. Cell nuclei were stained with DAPI. Western blot analysis demonstrated that the high expression levels of ERα, whereas low expression levels of ERβ and AQP2 were observed in glial cells compared to glioma cells (b, c). **P < 0.01 compared with the corresponding control. CK normal tissues, T tumor group, TL low-grade tumor, TH high-grade tumor