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. 2018 Oct 17;4:49. doi: 10.1038/s41420-018-0113-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — The expression of ANKFY1, LAX1, and LTBP1 in U87 cell lines was analyzed by western blot (a) and RT-qPCR (b–d). E2 decreased the expressions of ANKFY1, LTBP1, and LAX1 genes in nucleus of U87 cell line. Overexpression of AQP2 promoted the expressions of ANKFY1, LAX1, and LTBP1 genes, whereas it was attenuated by E2. Three independent experiments were repeated. **P < 0.01 vs. control, *P < 0.05 vs. control. NC normal cells, AQP2NCsiRNA overexpression of AQP2