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. 2018 Oct 17;4:49. doi: 10.1038/s41420-018-0113-y

Fig. 5. The pathway of E2 influences the localization of AQP2 in the U87 cell nucleus.

Fig. 5

Invasion of U87 cell was influenced by siRNA in relation to ANKFY1, LAX1, and LETP1 genes analyzed using the transwell assay (a–f). Overexpression of AQP2 decreased the cell invasion, while it was attenuated by siRNA in relation to ANKFY1, LAX1, and LETP1 genes. g showed that AQP2 + pGL3-LAX1 was loaded using HEK 293T vectors and transfected successfully to the U87 cell line. Luciferase reporter assays were performed. h, i Western blot and RT-qPCR showed LAX1 gene expression in the nucleus. AQP2 promoted LAX1 expression, which was attenuated by LAX1siRNA. j showed that siRNA ERα increased ANKFY1, LAX1, LETP1, and AQP2 mRNA levels and was further corroborated by the overexpression of ERβ condition analyzed by RT-qPCR (k). The results are expressed as the means ± SEM of three independent experiments. *P < 0.05, **P < 0.01 vs. control. Si siRNA, AQP2NCsiRNA overexpression of AQP2