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. 2018 Oct 17;8:15304. doi: 10.1038/s41598-018-33601-w

Figure 6.

Figure 6

Scheme depicting role of HORMAD1 in DSB signaling and repair. Our immunofluorescence microscopy analyses (Fig. 4) show that formation of CtIP, RAD51 and RPA p34-containing IRIF is reduced in HORMAD1-depleted cancer cells (whereas 53BP1 and p95/NBS1 foci remain intact in the absence of HORMAD1). We infer that HORMAD1 contributes to HR pathway activation at (or prior to) the CtIP-mediated resection step, but does not affect the proximal stages of DSB signaling. HORMAD1-depletion leads to attenuation of DR-GFP/HR reporter activity (Fig. 3), prolonged IR-induced ATM signaling (Fig. 4), and radiosensitivity (Fig. 3), further demonstrating that HORMAD1 promotes the HR pathway.