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. 2018 Oct 17;8:15306. doi: 10.1038/s41598-018-33550-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence staining of oocytes for ACACA, FASN, FADS1, SCD and TUBULIN distribution patterns assessment. NC – negative control (without primary antibodies). BF – brightfield, DAPI – chromatin staining and merged channels. All stacks present (excluding marked GV tubulin staining) oocytes at MII stage of meiosis (visible and stained polar body). Scale bars represent 50 μm.