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. 2018 Oct 17;8:15298. doi: 10.1038/s41598-018-33720-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Histological observation of Crassostrea gigas pediveliger larvae stained with hematoxylin and eosin (A–G) and multichrome technic (Alcian Blue, Periodic Acid–Schiff’s, Haematoxylin, Picric Acid) (H). A: Transverse, B: Frontal, C and H: Sagittal sections of the whole larvae. (D–G) show serial transverse sections of a foot. aam: anterior adductor muscle, bd: byssus duct, f: foot, fg: foot gland, fm: foot muscle, gr: gill rudiment, m: mantle, mo: mouth, o- oesophagus, p: periostracum, pam: posterior adductor muscle, pg: pedal ganglia, v: velum, vc: visceral cavity, arrow: secreting cells from the foot gland.