Fig. 5.

GLR-1 and GLR-5 currents gated by glutamate have different activation thresholds and kinetics. a Average traces of glutamate-evoked currents recorded in the AIB neurons of avr-14;glr-1 and avr-14;glr-5 double mutant background using perforated patch-clamp recording. A series of concentrations of glutamate (25 μM–1 mM) were perfused to the soma of AIB. AIB neurons were voltage clamped at −70 mV. n ≥ 5. b Quantification of the glutamate-induced currents in the AIB neurons of avr-14;glr-1 and avr-14;glr-5 double mutants, respectively. Error bars: s.e.m. n ≥ 5. c The normalized glutamate-induced currents in the AIB neurons of avr-14;glr-1 and avr-14;glr-5 double mutants were plotted as a function of the concentrations of glutamate. The tested glutamate concentrations were (in μM): 25, 50, 100, and 1000. Data were fit with the Hill equation: I/I _max = 1/[1 + (EC₅₀/[glutamate])ⁿ], where n represents Hill slope (Hill coefficient)