Fig. 3.

MINA53 deficiency induces DNA damage and reduces the activity of the ATM/ATR-H2AX pathway. a Representative fluorescent micrographs of comet assays and b quantification of the percentage of tail DNA of the indicated cells. Scale bar = 15 μm. c, d Immunofluorescence staining of γ-H2AX and quantification of γ-H2AX-positive cells of the indicated cells. Scale bar = 5 μm. e Western blot analysis of the indicated proteins in LN-229 and U-87 MG cells with or without MINA53 knockdown. All data were analyzed using two-tailed Student’s t-tests. Mean ± s.e.m., n = 3, ***P < 0.001