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. 2018 Oct 17;8:15313. doi: 10.1038/s41598-018-33575-9

Table 1.

Primers used in the present study for RT-PCR analysis.

Name Direction Sequence (5′ → 3′) Tm (°C)
Oct-04 Sense AGT GAG AGG CAA CCT GGA GA 54
Antisense GTG AAG TGA GGG CTC CCA TA
Nanog Sense TTC TTG ACT GGG ACC TTG TC 54
Antisense GCT TGC CTT GCT TTG AAG CA
Sox-2 Sense AGA ACC CCA AGA TGC ACA AC 52
Antisense GGG CAG CGT GTA CTT ATC CT
NF-68 Sense GAG TGA AAT GGC ACG ATA CCT A 58
Antisense TTT CCT CTC CTT CTT CTT CAC CTT C
Cardiac Sense GGA GTT ATG GTG GGT ATG GGT C 58
Antisense AGT GGT GAC AAA GGA GTA GCC A
AFP Sense ATG CTG CAA ACT GAC CAC GC 55
Antisense GCT TCG CTT TGC CAA TGC TT
HLA-G Sense GCG GCT ACT ACA ACC AGA GC 58
Antisense GCA CAT GGC ACG TGT ATC TC
β-actin Sense TCC TTC TGC ATC CTG TCA GCA 58
Antisense CAG GAG ATG GCC ACT GCC GCA
Nanos3 Sense CTC TGC ATG AGG AAG AGG AGC C 60
Antisense GGA CTG ATA GAT CGC ACG AGA
Nobox Sense AGC CAG TGC AGA TCT GCA CC 60
Antisense TGT CAC TGC CAG GAA CAT CCC TC
Lhx8 Sense GTA TCA CTT GGC TTG CTT 60
Antisense ATT ACC GTT CTC CAC TTC
GAPDH Sense GGA AAG CTG TGG CGT GAT 60
Antisense AAG GTG GAA GAA TGG GAG TT