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. 2018 Oct 17;9:4308. doi: 10.1038/s41467-018-06767-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Septin ring splitting and CAR constriction are spatially and temporally separated events. a Logarithmically growing cells expressing Shs1-mCherry and Myo1-GFP were fixed and processed for SIM. The image shows an example of split septin rings sandwiching the CAR. Scale bar: 2 μm. Graphs show the quantification of fluorescence intensities along the yellow dotted line in the merge. Dotted red line: Shs1-mCherry; green line: Myo1-GFP. A.U.: Arbitrary Units. b Same cells as in a were imaged live every min through their cell cycle. Quantification of fluorescence intensities associated to Shs1-mCherry and Myo1-GFP around the time of septin ring splitting (time 0). Fluorescence intensity associated to septin and myosin II has been quantified by ImageJ in cells undergoing cytokinesis (graph; red squares: Shs1-mCherry; green circles: Myo1-GFP) and then related to the highest fluorescence intensity of each structure in a given cell. Plots show average values (n = 15). Error bars: s.d. Cropped images beneath the graph show the behavior of septin ring and CAR during this time frame in one representative cell. Shs1 was pseudocolored with the Fire plugin of Image J to reflect signal intensity (orange/red signals mean higher fluorescence intensity than magenta signals)