Fig. 2.

The MEN factors Tem1, Cdc15, and Mob1 are required for septin ring splitting and CAR contraction independently of mitotic exit. a, c, e Cells with the indicated genotypes were grown in permissive conditions and then shifted to restrictive conditions 60–90 min prior to imaging. Cells were filmed every 2 min (a) or 4 min (c, e) for 4–8 h in restrictive conditions (a glucose-containing medium; c medium supplemented with 5 µM 1NM-PP1; e 32 °C). Arrowheads indicate the appearance of new septin rings (yellow) or CARs (white) before the old structures have been disassembled. DIC differential interference contrast. TL transmitted light. Scale bar: 5 µm. b, d, f Cells with the indicated genotypes were grown in permissive conditions (b YEPRG; d, f YEPD) at 25 °C, arrested in G1 with alpha factor and then released in restrictive conditions (b YEPD; d YEPD containing 5 µM 1NM-PP1; f YEPD at 32 °C). At various time points after release (time 0) cells were collected for FACS analysis of DNA contents. FACS data were plotted after gating out the debris as illustrated in Supplementary Fig. 12