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. 2018 Oct 17;9:4308. doi: 10.1038/s41467-018-06767-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Septin destabilization triggers CAR contraction in GAL1-DMA2 cells. a, c GAL1-DMA2 BUD4 cells with the indicated genotypes and expressing Shs1-mCherry and Myo1-GFP were grown in SD-raffinose and induced for 90 min with galactose before being mounted with SD raffinose/galactose for imaging at 30 °C (every 2 min for 2 h). Arrowheads indicate disassembly of septin rings (yellow) or the onset of CAR constriction (white). DIC differential interference contrast. b GAL1-DMA2 BUD4 shs1Δ cells expressing either Cdc10-eGFP (upper panel) or Myo1-GFP (bottom panel) were treated as in a. Scale bar: 5 μm. d Quantification of fluorescence intensities associated to Shs1-mCherry and Myo1-GFP around the time of septin ring splitting (time 0) in GAL1-DMA cdc12-1 (n = 10) and GAL1-DMA2 TEM1-Q79L cells (n = 13): red squares: Shs1-mCherry; green circles: Myo1-GFP. Error bars: s.d. e Cells with the indicated genotypes and expressing Shs1-mCherry and Myo1-GFP were induced with galactose for ~90 min and imaged every 2 min for 2 h at 30 °C in SD-raffinose/galactose. Cells were classified according to their behavior for what concerns septin ring splitting and CAR constriction