Fig. 6.

Constitutive association between Dma2 and Nud1 prevents mitotic exit and cytokinesis. a Meiotic segregants obtained after sporulation of diploid cells generated by crossing NUD1-GBD with DMA2-eGFP haploid cells. Genotypes were confirmed by PCR. b Serial dilutions of cells with the indicated genotypes were spotted on YEPD and YEPG plates and incubated at 30 °C. c–e NUD1-GBD GALs-DMA2-eGFP cells, either BUD4 or bud4-G2459fs, expressing Shs1-mCherry and grown in SD-raffinose were induced for 90 min with galactose and then imaged in SD-raffinose/galactose at 30 °C every 4 min. c Telophase arrest; d cytokinesis defects; e number of cells showing the indicated phenotypes in the movies. Arrowheads indicate Dma2-eGFP at SPBs. TL transmitted light. Scale bar: 5 µm