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. 2018 Oct 11;9:2347. doi: 10.3389/fimmu.2018.02347

Figure 5.

Figure 5

Checkpoint blockade reinvigorates parasite-specific CD8+ T cell immunity and enhances parasite control in infected IL-17RA KO mice. (A) Layout of the checkpoint blockade experiment. (B) Representative plots and statistical analysis of CD8 and TSKB20/Kb staining in spleen and liver of the indicated experimental groups. Numbers within plots indicate the frequency of total CD8+ cells (up) and TSKB20-specific CD8+ T cells (down) at 21 dpi. Bar graphs represent data as mean ± SD, N = 7 mice. (C) Relative amount of T. cruzi satellite DNA in the spleens of the indicated experimental groups at 21 dpi. Murine GAPDH was used for normalization. Data are presented as mean ± SD relative to WT-cAb group, N = 7 mice. (D) Enzymatic activity (UI/L) of alanine transaminase (ALT), aspartate transaminase (AST), creatinine kinase-MB and creatinine phosphokinase (CPK) on plasma of the indicated experimental groups at 21 dpi. N = 7 mice. All P values were calculated with two-tailed T test. Data are representative of two independent experiments.