Figure 2.

Serum IgGs react with antigens expressed on axons and disorganized myelin. IgGs from SCI individuals (in red) bind to normal appearing axons (NF-positive, in white) in control rat spinal cords (A–D) as well as to dystrophic axons in injured tissue (E–H). However, IgGs do not seem to react with normally appearing myelin (as detected by being MBP-positive, in green), although they can be localized in some disorganized myelin structures in SCI tissue (yellow signal in G, H). All images are a single confocal plane. NF, neurofilament. MBP, myelin basic protein. Scale bar, 50 μm.