Table 1.
Messenger RNA levels in cultured CCL-136 cells treated for 24 h.
| Cytokines | Added NaCl | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| IFNγ | IL1β | TNFα | IFNγ+IL1β | IFNγ+TNFα | IL1β+TNFα | 25 mM | 50 mM | 75 mM | 100 mM | |
| SLC5A3 | 1 | 1.1 | 1.3 | 2.1 | 4.6 | 10.3 | 0.6 | 0.5 | 1.9 | 3.4 |
| SLC6A6 | 0.5 | 0.7 | 0.8 | 1.4 | 1.5 | 4.3 | 0.9 | 1.1 | 1.2 | 1.5 |
| SLC6A12 | 1.7 | 2 | 1 | 3.4 | 3.8 | 1.2 | 1.6 | 7.7 | 9 | 178.7 |
| AKR1B1 | 1.4 | 2.3 | 1.9 | 1.9 | 1.6 | 1.7 | 1.4 | 2.1 | 2.4 | 5.6 |
| NFAT5 | 1.1 | 0.3 | 0.5 | 1.4 | 1.7 | 1 | 1.4 | 4.1 | 0.8 | 2.5 |
| MAPK14 | 0.7 | 0.8 | 0.8 | 1.4 | 1.9 | 1.4 | 1.1 | 2 | 0.9 | 1.3 |
| RELA | 0.9 | 1.2 | 0.9 | 1.8 | 1.7 | 0.5 | 0.9 | 0.8 | 0.9 | 1.2 |
| NFkB1 | 1 | 1.2 | 1 | 6 | 0.5 | 3.8 | 1.5 | 2.9 | 1.2 | 1.9 |
| NFkB2 | 1.3 | 1.5 | 2.7 | 3.3 | 12.9 | 1 | 1.2 | 2.3 | 1.5 | 1.1 |
Fold changes compared to untreated cells, normalized to glyceraldehyde 3-phosphate dehydrogenase expression levels, were obtained through quantitative reverse transcription PCR and calculated as 2−ΔΔCt (mean of three given). Fold changes >2 green; >5 yellow; >10 orange; >100 red.