Figure 3.

Characterization of the DTBP platform for simple and low‐cost cfNA isolation. A) The DTBP platform consists of several microwells in a single microfluidic chip. The width, depth, and length were optimized for cfNA isolation. B) The amplification efficiency of this platform is dependent on the type of homobifunctional imidoester reagents (dimethyl adipimidate (DMA), dimethyl suberimidate (DMS), dimethyl pimelimidate (DMP), and DTBP). The cfDNA capture rate is dependent on the C) DTBP concentration (mg mL⁻¹) and D) APDMS concentration (µL mL⁻¹). The error bars indicate the standard deviation from the mean, based on at least three independent experiments. E) cfRNA isolation from blood plasma using the DTBP platform. (M: size marker, 1: plasma #1, 2: plasma #2, N: negative control).