Figure 5.

A) Representative confocal microscopic images of annexin V‐FITC/PI double stained U87 cells treated with LysoIr@PDA‐CD‐RGD (20 µg mL⁻¹, 12 h) in the absence and presence of light. FITC: λ_ex = 488 nm; λ_em = 530 ± 20 nm; PI: λ_ex = 514 nm; λ_em = 620 ± 20 nm. B) Detection of caspase 3/7 activity in U87 cells treated with LysoIr@PDA‐CD‐RGD at the indicated concentrations in the absence or presence of light. C) Detection of cellular ATP content. D) Observation of cathepsin B release from lysosomes to cytosol in U87 cells treated with LysoIr@PDA‐CD‐RGD in the absence or presence of the 808 laser irradiation. λ_ex = 543 nm; λ_em = 630 ± 20 nm. E) Visualization of lysosomal pH in U87 cells. The cells were treated with LysoIr@PDA‐CD‐RGD (20 µg mL⁻¹, 4 h) and stained with Lyso‐DR (1 µg mL⁻¹, 30 min). After irradiation, the cells were imaged with a confocal microscope at 0, 30 and 60 min. Dansyl: λ_ex = 405 nm; λ_em = 440 ± 30 nm. R6G‐amide: λ_ex = 543 nm; λ_em = 590 ± 30 nm. For the light‐treated samples in (A)‒(E), cells were irradiated with an 808 nm laser at a light dose of 1 W cm⁻² for 5 min. Scale bar: 10 µm.