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. 2018 Sep 20;90(20):11835–11846. doi: 10.1021/acs.analchem.8b01378

Figure 2.

Figure 2

Multimodal imaging of healthy human liver tissue. (I) Optical image of tissue section prior to matrix application or staining. (II) Tissue sections stained with hematoxylin and eosin and (III) Masson’s trichrome stain. Negative-ion mode (A) MALDI-MS and (B) MALDI-FTICR-MS images showing the distribution of selected molecular species in the (IV) bile duct lumen ([TCA – H]− at m/z 514.2849); (V) connective tissue ([PA (18:0_18:1) – H]− at m/z 701.5128); (VI) parenchyma ([PI (18:0_20:4) – H]− at m/z 885.5504); and (VII) bile duct ([ST–OH (18:1_24:0) – H]− at m/z 906.6339). (VIII) Overlay of the selected species. (Designated masses are from MALDI-FTICR-MS imaging measurements, MALDI-MS image field of view 78624 pixels, area 110 mm2, and spatial resolution 50 μm. MALDI-FTICR-MS image field of view 24915 pixels, 5.5 mm2 area, and spatial resolution 15 μm. Images normalized with TIC).