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. 2018 Sep 20;90(20):11835–11846. doi: 10.1021/acs.analchem.8b01378

Figure 4.

Figure 4

Multimodal imaging of human liver from patients with (A) mild PSC and (B) severe PSC. (I) Optical image of tissue section prior to matrix application or staining. Consecutive tissue sections stained with (II) hematoxylin and eosin (III) Masson’s trichrome. Negative-ion mode MALDI-FTICR-MS images showing the distribution of (IV) [TCA – H]− at m/z 514.2855; (V) sulfated bile alcohol ([M – H]−) at m/z 531.2999; (VI) bilirubin diglucuronide ([M – H]−) at m/z 935.3218; (VII) heme ([M – H]−) at m/z 615.1704; (VIII) [PA (18:0_18:1) – H]− at m/z 701.5128; (IX) [PI (18:0_20:4) – H]− at m/z 885.5504; and (X) [ST–OH (18:1_24:0) – H]− at m/z 906.6353. (XI) Overlay of the selected species. (Mild PSC image field of view 20326 pixels and area 10 mm2, severe PSC image field of view 63744 pixels, and area 14 mm2. Spatial resolution of both images 15 μm, normalized with TIC).