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. Author manuscript; available in PMC: 2019 Oct 4.
Published in final edited form as: Mol Cell. 2018 Sep 20;72(1):140–151.e3. doi: 10.1016/j.molcel.2018.09.001

Figure 4. Deletion of CTF4 impairs the transfer of parental H3-H4 to lagging strands.

Figure 4.

(A) Snapshot of BrdU-IP-ssSeq, H3K56ac and H3K4me3 eSPAN peaks at ARS1309 in ctf4Δ mutant cells. (B-E) Analysis of the average bias ratio and pattern of H3K56ac (B-C) and H3K4me3 (D-E) eSPAN peaks in ctf4Δ mutant cells. H3K4me3 eSPAN peaks in ctf4Δ mutant cells show a strong leading-strand bias. Data in C and E are represented as mean ± SEM from two independent experiments. (F) Analysis of the relative levels of H3K4me3 at leading and lagging strands of newly replicated chromatin in ctf4Δ mutant cells compared to wild type cells. The representation and calculation methods are the same as in Figure 1F. (G) The effect of mcm2-3A mutation on H3K4me3 eSPAN peaks is highly correlated to that of ctf4Δ cells. One dot represents the bias ratio of eSPAN peaks at individual nucleosomes (±10 nucleosomes) surrounding each of 134 early DNA replication origins. See also Figure S3.