Table 1.
Summary of the CRISPR-induced exon skipping in rabbits
| Mutation type | Rabbits lines | Nature of mutation | Predicted ESS | PTC in exon | Exon skipping | Method |
|---|---|---|---|---|---|---|
| Non-frameshift | D3 (Additional file 2: Figure S1) | − 75 bp in exon 51 | Yes | No | No | CRISPR/Cas9 |
| L2 (Additional file 2: Figure S2) | (− 4, + 1) bp in exon 2 | Yes | No | No | CRISPR/Cas9 | |
| K2 (Additional file 2: Figure S6) | WT/− 6 bp in exon 3 | Yes | No | No | CRISPR/Cas9 | |
| K3 (Additional file 2: Figure S6) | − 6 bp in exon 3 | Yes | No | No | CRISPR/Cas9 | |
| Missense | T2 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 |
| T3 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
| T4 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
| T5 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
| T6 (Additional file 2: Figure S7) | C>T in exon 14 | No | No | No | BE3 | |
| PTCs | D2 (Additional file 2: Figure S1) | − 157 bp/− 70 bp in exon 51 | Yes | Exon 51 | Exon 51 (233 bp) | CRISPR/Cas9 |
| L3 (Additional file 2: Figure S2) | − 106 bp/− 14 bp in exon 2 | Yes | Exon 2 | Exon 2 (157 bp) | CRISPR/Cas9 | |
| A2 (Additional file 2: Figure S3) | − 10 bp/− 13 bp in exon 12 | Yes | Exon 12 | Exon 12 (223 bp) | CRISPR/Cas9 | |
| G1 (Additional file 2: Figure S4) | − 91 bp in exon 5 | Yes | Exon 5 | Exon 5 (130 bp) | CRISPR/Cas9 | |
| G2 (Additional file 2: Figure S4) | − 14 bp in exon 5 | Yes | Exon 5 | Exon 5 (130 bp) | CRISPR/Cas9 | |
| G3 (Additional file 2: Figure S4) | − 5 bp/− 91 bp in exon 5 | Yes | Exon 5 | Exon 5 (130 bp) | CRISPR/Cas9 | |
| G4 (Additional file 2: Figure S4) | − 5 bp in exon 5 | Yes | Exon 5 | Exon 5(130 bp) | CRISPR/Cas9 | |
| B3 (Additional file 2: Figure S5) | C>T in exon 20 | Yes | Exon 20 | Exon 20 (242 bp) | BE3 | |
| B4 (Additional file 2: Figure S5) | C>T in exon 20 | Yes | Exon 20 | Exon 20 (242 bp) | BE3 | |
| PTCs in exon 1 | M2 (Additional file 2: Figure S8) | C>T in exon 1 | No | Exon 1 | No | BE3 |
| M3 (Additional file 2: Figure S8) | C>Tin exon 1 | No | Exon 1 | No | BE3 | |
| Y2 (Additional file 2: Figure S9) | C>T in exon 1 | No | Exon 1 | No | BE3 | |
| Y3 (Additional file 2: Figure S9) | C>T in exon 1 | No | Exon 1 | No | BE3 |
The ESS sites were predicted by online tool (http://astlab.tau.ac.il/index.php)
−, deletion; +, insert; BE3, Cytidine base editors; ESS, essential splice site
D1, D2, D3, the DMD gene-edited rabbits by CRISPR/Cas9; L1, L2, L3, the LMNA gene-edited rabbits by CRISPR/Cas9; K1, K2, K3, the GCK gene-edited rabbits by CRISPR/Cas9; A1, A2, the ANO5 gene-edited rabbits by CRISPR/Cas9; G1, G2, G3, G4, G5, the GHR gene-edited rabbits by CRISPR/Cas9; B1, B2, B3, B4, the DMD gene-edited rabbits by BE3; T1, T2, T3, T4, T5, T6, the TIA1 gene-edited rabbits by BE3; M1, M2, M3, the MSTN gene-edited rabbits by BE3; Y1, Y2, Y3, the TYR gene-edited rabbits by BE3