TABLE 1.
GC-MS data for the metabolites of naphthalene, phenanthrene, and pyrenea
| Substrate | Retention time (min) | m/z of major ion peaks (%) | Suggested metabolite |
|---|---|---|---|
| Pyrene | 44.576 | 236 (M+, 100), 200 (38), 174 (8), 150 (6), 118 (12), 100 (32), 74 (7), 4 (12) | 4,5-Dihydroxypyrene |
| 48.365 | 254 (M+, 38), 236 (21), 221 (100), 202 (31), 167 (45), 149 (27), 128 (33), 113 (18), 96 (54), 40 (70) | Phenanthrene-4,5-dicarboxylic acid | |
| 38.683 | 208 (M+, 100), 193 (86), 176 (23), 164 (24), 150 (26), 132 (19), 118 (22), 105 (44), 79 (62), 51 (29) | 3,4-Dihydroxyphenanthrene | |
| 46.365 | 279 (M+, 23), 167 (37), 149 (100), 132 (13), 113 (15), 71 (35), 57 (34), 43 (28) | 2-Carboxy-1-naphthol | |
| 18.643 | 161 (M+, 100), 146 (65), 120 (36), 94 (53), 69 (31) | 1,2-Naphthalenediol | |
| 7.893 | 122 (M+, 100), 121 (90), 104 (22), 93 (38), 76 (24), 65 (33) | Salicylaldehyde | |
| 9.578 | 138 (M+, 72), 120 (100), 92 (64), 64 (33) | Salicylic acid | |
| 8.751 | 110 (M+, 100), 92 (28), 81 (18), 64 (35), 63 (19) | Catechol | |
| Phenanthrene | 38.175 | 208 (M+, 100), 193 (88), 176 (30), 164 (17), 150 (28), 132 (12), 118 (24), 105 (33), 79 (59), 51 (13) | 3,4-Dihydroxyphenanthrene |
| 47.024 | 279 (M+, 43), 167 (24), 149 (100), 132 (22), 113 (12), 71 (24), 57 (37), 43 (16) | 2-Carboxy-1-naphthol | |
| 18.594 | 161 (M+, 100), 146 (35), 120 (28), 94 (34), 69 (14) | 1,2-Naphthalenediol | |
| 7.605 | 122 (M+, 100), 121 (77), 104 (31), 93 (27), 76 (26), 65 (36) | Salicylaldehyde | |
| 9.604 | 138 (M+, 57), 120 (100), 92 (55), 64 (20) | Salicylic acid | |
| 8.816 | 110 (M+, 100), 92 (41), 81 (25), 64 (17), 63 (20) | Catechol | |
| Naphthalene | 19.034 | 161 (M+, 100), 146 (46), 120 (31), 94 (20), 69 (11) | 1,2-Naphthalenediol |
| 7.506 | 122 (M+, 100), 121 (66), 104 (24), 93 (30), 76 (19), 65 (22) | Salicylaldehyde | |
| 9.742 | 138 (M+, 38), 120 (100), 92 (64), 64 (15) | Salicylic acid | |
| 8.542 | 110 (M+, 100), 92 (50), 81 (10), 64 (28), 63 (37) | Catechol |
a
The metabolites were obtained from the organic extracts of the cultures and resting cell incubations of Cycloclasticus sp. P1.