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. Author manuscript; available in PMC: 2019 Oct 19.
Published in final edited form as: J Mol Biol. 2018 Sep 1;430(21):4307–4324. doi: 10.1016/j.jmb.2018.08.025

Figure 6: Lack of MMTV full length gRNA in the nucleus suggests defects in transcription.

Figure 6:

qPCR analysis of wild type (WT) full-length gRNA up to 48 hours after Actinomycin D treatment in: (A) nuclear (NUC), (B) cytoplasmic (CYT), and (C) total cDNAs prepared from HEK293T cells transiently transfected with the indicated clones. Full-length gRNA expression was quantified using the custom-made Taqman Assay 2 that binds between nt 1729–1791 of HYB-MTV genome, located at the 5’ end of the gag gene (see Fig. 1 and Table 1 for details).