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. Author manuscript; available in PMC: 2019 Aug 29.
Published in final edited form as: J Am Chem Soc. 2018 Aug 17;140(34):10749–10756. doi: 10.1021/jacs.8b04238

Figure 2.

Figure 2.

TpnL-mediated conversion of thiostrepton A (1) to thiostrepton Aa (7). (A) HPLC analyses of culture extracts from (a) S.laurentii, (b) S. laurentii HI0, (c) S. laurentii HI1, (d) coinjection of S. laurentii and HI1 extracts, and (e) coinjection of S. laurentii HI0 and HI1 extracts. (B) HPLC analyses of TpnL activity. Reactions omitting TpnL are shown at the top and reactions including TpnL are shown at the bottom. Reaction mixtures were incubated for (i) 0 min, (ii) 10 min, (iii) 60 min, and (iv) 6 h. Absorbance was monitored at 254 nm. 1 is represented by ○ and 7 by ●.