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. Author manuscript; available in PMC: 2018 Oct 18.
Published in final edited form as: Eur J Pharmacol. 2008 Feb 5;584(2-3):213–221. doi: 10.1016/j.ejphar.2008.01.036

Fig. 1.

Fig. 1.

Purification of recombinant wild-type and C298S mutant AKR1B10. Lane assignments are as follows: 1) protein marker, 2) extract of whole cells before induction, 3) soluble proteins after cell lysis, 4) column flow-through fraction, 5) column wash fraction, 6) eluted AKR1B10, wild-type, 7) marker proteins and 8) eluted C299S AKR1B10 mutant.