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. 2018 Oct 11;9:2287. doi: 10.3389/fmicb.2018.02287

FIGURE 5.

FIGURE 5

The flagella- and H1-T6SS-related genes of PA are significantly differentially expressed by the presence of KP during evolution. (A) Comparison of the global transcriptional profiles of PA between monoevolution of PA and PA-KP coevolution. Total RNAs of each culture were isolated at cycle 21 (day 21). The up- and downregulated genes were separated by the dashed line. Differential expression of (B) flagella-related genes, (C) H1-, (D) H2-, and (E) H3-T6SS related genes of PA when PA was cultured in different conditions. 3 Mixture-PA C21, coevolution of PA, SA, and KP compared with monoevolution of PA (cycle 21); KPPA-PA C1, coculture of KP and PA compared with monoculture of PA (cycle 1); KPPA-PA C21, coevolution of KP and PA compared with monoevolution of PA (cycle 21); PA-SAPA C21, monoevolution of PA compared with coevolution of SA and PA (cycle 21); CS-LS C1, monoculture of PA in M9-casamino acids broth at the 24 h time point compared to that in M9-PCh broth. ∗FDR < 0.05.