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. Author manuscript; available in PMC: 2018 Oct 18.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Dec 22;495(3):2257–2263. doi: 10.1016/j.bbrc.2017.12.127

Figure 2: anos1 is a target of Pax3 and Zic1.

Figure 2:

(A) Injection of pax3MO (40ng) or zic1MO (40ng) reduces anos1 expression at the neurula stage. Dorsal views, anterior to top. The injected side is to the right as indicated by the presence of the lineage tracer (Red-Gal). The graph indicates the percentage of embryos with normal (white) or reduced/lost (red) anos1 expression. The number of embryos analyzed is indicated on top of each bar. (B) mRNA encoding pax3GR and zic1GR (100pg each), alone or in combination were injected into both blastomeres in the animal pole at the 2-cell stage. At the blastula stage (stage 9), animal cap (AC) explants were dissected and cultured for 8 hours in the presence of dexamethasone (+dex). (C) anos1 and snai2 expression in pax3GR and zic1GR injected AC explants analyzed by qRT-PCR.