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. Author manuscript; available in PMC: 2018 Oct 18.
Published in final edited form as: Biochem Biophys Res Commun. 2017 Dec 22;495(3):2257–2263. doi: 10.1016/j.bbrc.2017.12.127

Figure 4: Anos1 is essential for the formation of cranial placodes and their derivatives.

Figure 4:

(A-D) Unilateral injection of anos1MO (40ng) at the 2-cell stage causes a reduction/loss of expression of two pan-placodal genes, six1 (A) and foxi4.1 (B), as well as genes restricted to individual placodal domains such as dmrta1 (C) and pax8 (D). (E-G) Later in development, the injected embryos exhibited reduce dmrta1 (E), emx2 (F) and ebf2 (G) expression in the olfactory epithelium. (H-I) The expression of foxe1 in the developing pituitary was also reduced (I) as compared to sibling control (H). (A-I) Anterior views, dorsal to top. The injected side (arrowheads) is to the right. The percentage of affected embryos is indicated in the upper right corner of each panel.