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. 2018 Oct 11;9:471. doi: 10.3389/fpsyt.2018.00471

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Copyright © 2018 Wang, Wang, Xu, Liu, Jiang and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CUMS induced M1 marker iNOS expression in the prefrontal cortex and hippocampus of both adolescent and adult rats, while minocycline treatment reduced the expression. (A) iNOS expression in the prefrontal cortex and hippocampus in immunohistochemistry (× 400); (B) iNOS-positive cell counts in the prefrontal cortex; (C) iNOS-positive cell counts in the hippocampus; (D) Western blot analysis of iNOS in the prefrontal cortex; (E) Western blot analysis of iNOS in the hippocampus. Results are expressed as the mean ± SEM (n = 4 each group; n = 5 each group). ^#, ^##, and ^### indicate p < 0.05, p < 0.01, and p < 0.001 vs. C group, respectively; ^*, ^**, and ^*** indicate p < 0.05, p < 0.01, and p < 0.001 vs. S group, respectively. AdoC, adolescent control group; AdoS, adolescent CUMS group; AdoS+M, adolescent CUMS and minocycline group; AduC, adult control group; AduS, adult CUMS group; AduS+M, adult CUMS and minocycline group.