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. Author manuscript; available in PMC: 2018 Oct 18.
Published in final edited form as: Mol Cell. 2018 Sep 27;72(1):178–186.e5. doi: 10.1016/j.molcel.2018.08.033

Figure 5. A designed repertoire of acyl-CoA-synthetases and substantially broadened substrate selectivity.

Figure 5

(A) The ACS active site. Positions where design was enabled are shown as green sticks, and AMP and CoA substrates are shown as teal lines. Amino acid positions and identities allowed during design are indicated. (B) Specific activity of ACS variants, normalized to that of ACS_PROSS with acetate, demonstrates substantial broadening of substrate selectivity in designs ACS_9, ACS_16, and ACS_27. See also Tables S5-S6 and Figures S4-S5.