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. Author manuscript; available in PMC: 2019 Apr 15.
Published in final edited form as: Cancer Res. 2018 Aug 2;78(20):5741–5753. doi: 10.1158/0008-5472.CAN-18-0655

Figure 1. Specific microRNAs are associated with translating polysomes.

Figure 1

A) Diagram of the high-throughput analysis of miRNA expression on the translational machinery of REN cells. We performed a sucrose density gradient sedimentation in both EDTA and EDTA-free conditions, followed by microRNA profiling. B) Heat map with microRNAs expressed along the polysomal profile of REN cells, in the absence of EDTA. C) miRNA polysome association following EDTA disruption: a limited set of miRNAs is very sensitive to EDTA condition (light blue), indicating that they associate dynamically with polysomes. Most miRNAs analyzed are less sensitive to EDTA (blue and dark blue), suggesting that they are not active on polysomes, but may rather cosediment with other high molecular weight structures.