Fig 2. Picture showing setup and transfer syringes used for production and purification of ²¹¹At-BC8-B10.

Reagent vials containing (1) ammonium acetate, (2) BC8-B10, (3) sodium thiosulfate, and (4) sodium ascorbate are not shown. Those reagents were added via syringe to the reaction vial containing ²¹¹At. Once the additions were made, the reaction solution was taken up into the large syringe and 1/3 volume was added to the top of each column. Following that the pre-rinsed columns were sequentially eluted with 4 mL of sterile PBS/sodium ascorbate solution. Fractions containing the ²¹¹At-BC8-B10 were collected in the product vessel and other elution volumes were directed to the waste vessel.