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. 2018 Oct 18;13(10):e0204571. doi: 10.1371/journal.pone.0204571

Fig 2. Effect of vitrification on the mitochondrial DNA integrity of embryos immediately after warming.

Fig 2

The integrity of the mitochondrial genome was assessed according by the ratio of the Mt-number (long sequence/short sequence) predicted by real-time PCR targeting short and long mitochondrial sequences. A) Relative Mt-number predicted by PCR targeting the short and long mitochondrial sequences; the relative value for fresh embryos was taken as 1.0. B) Ratio of the Mt-numbers (Mt-number predicted by PCR targeting long sequence/Mt-number predicted by PCR targeting short sequence PCR). Value for fresh embryos was taken as 1.0. Bars with different letters represent differences between treatments (P < 0.05). Data are presented as mean ± SEM.