Fig 5. Effect of resveratrol on the ROS content in blastocysts and blastomeres.

Vitrified-warmed embryos were cultured with either vehicle (control) or resveratrol (0.5μM), and the blastocysts were stained with CellROX. A) ROS content per blastocyst, B) ROS content per blastomere, C) Representative images of CellROX-stained blastocysts. The average fluorescence intensity of vitrified-warmed embryos cultured with vehicle (control) was defined as 1.0. Bars with different letters represent difference between treatments (P < 0.05). Data are presented as mean ± SEM.