Table 1. Details of primers, annealing temperature and the obtained product size of the amplification of gene targets in this study.
Region | Primer name | Orientation | Reference | Annealing temperature (°C) | Obtained product size (bp) |
---|---|---|---|---|---|
ITS | ITS5 | Forward | [35] | 54 | 589 |
ITS4 | Reverse | ||||
ACT | ACT512F | Forward | [36] | 58 | 285 |
ACT783R | Reverse | ||||
TUB2 | T1 | Forward | [37] | 52 | 743 |
T22 | Reverse | ||||
GAPDH | GSF1 | Forward | [38] | 60 | 278 |
GSR1 | Reverse | ||||
CHS-1 | CHS-79F | Forward | [36] | 50 | 297 |
CHS-345R | Reverse |