Table 1. Descriptive information for the 10 microsatellite loci isolated from Pinus albicaulis.
Locus name | Primer sequence (5'–3') | Repeat motif | Allele size (bp) |
---|---|---|---|
ALBI_B08 | F: CGTTACAGTAATTGGACG | (CG)n | 259–271 |
R: GTTTGTAGCAAACAGTAC | |||
ALBI_063 | F: TCTCTTCGAGATTTAAACCCA | (AT)n | 197–201 |
R: ACCGTTCAGAATTAGACCAT | |||
ALBI_069 | F: GATCTCCGACCGTAATGTGT | (AG)n | 195–197 |
R: AGTGCGACCTTACAAAGAGG | |||
ALBI_112 | F: ATTTCCCACCATCCCTAATG | (TC)n | 284–325 |
R: GCGGACATACGGATCAAGT | |||
ALBI_113 | F: ATGAGGACCAGGAGTAAAT | (AG)n | 273–276 |
R: GCTATTGTGTAAGCCTGCGT | |||
ALBI_116 | F: AGAACATGAAAGGCCTAGGAA | (AT)n | 157–159 |
R: GGATCCTCTGGCCAAGTTAG | |||
ALBI_149 | F: TAGGATCCCTTCTTTTTGGG | (AC)n | 199–226 |
R: TTATGTCTTGGCTTGGCAGT | |||
ALBI_151 | F: GAACTTCTGAGGAAGCTTGATG | (AG)n | 167–197 |
R: TTCTGATCAATCTAAAGGTCAATCT | |||
ALBI_160 | F: TGTTATTGCTAACGGAGATGG | (TG)n | 229–233 |
R: TTTTCTATTGTAGACAGTGTCGTCTT | |||
ALBI_171 | F: ACAAAGCCACGAACAATCA | (CT)n | 267–271 |
R: GCCAATTGTTTGTTGGCTCTTTTAAC |
Locus name, primer sequences, repeat motif, and allele size range for the 10 microsatellite loci isolated for Pinus albicaulis Engelm. (Pinaceae). Annealing temperature is 58°C for all loci.