. 2018 Oct 18;13(10):e0205423. doi: 10.1371/journal.pone.0205423

Table 2. Microsatellite loci, source species, and application to two populations of Pinus albicaulis in the Greater Yellowstone Ecosystem.

Locus name	Source species	N_a			Henderson Mountain						Mt. Washburn
Locus name	Source species	N_a	H_o	H_e	N_A	A_P	A_N	H_O	H_E	HWE	N_A	A_P	A_N	H_O	H_E	HWE
ALBI_B08	P. albicaulis	2	0.025	0.025	2	1	0.0	0.045	0.044	ns	1	0	0.3	0.000	0.000	-
ALBI_063	P. albicaulis	3	0.500	0.404	3	1	0.0	0.455	0.361	ns	2	0	0.0	0.550	0.439	ns
ALBI_069	P. albicaulis	3	0.075	0.073	3	2	0.0	0.150	0.141	ns	1	0	0.0	0.000	0.000	-
ALBI_112	P. albicaulis	3	0.132	0.169	2	0	0.0	0.105	0.100	ns	3	1	0.2	0.158	0.234	ns
ALBI_113	P. albicaulis	3	0.231	0.207	3	1	0.0	0.143	0.135	ns	2	0	0.0	0.333	0.278	ns
ALBI_116	P. albicaulis	2	0.024	0.024	2	1	0.0	0.048	0.046	ns	1	0	0.0	0.000	0.000	-
ALBI_149	P. albicaulis	9	0.370	0.360	6	2	0.0	0.346	0.308	ns	7	3	0.0	0.400	0.423	ns
ALBI_151	P. albicaulis	2	0.023	0.023	2	1	0.0	0.043	0.043	ns	1	0	0.0	0.000	0.000	-
ALBI_160	P. albicaulis	3	0.319	0.354	3	0	0.0	0.407	0.381	ns	3	0	0.1	0.200	0.304	ns
ALBI_171	P. albicaulis	3	0.150	0.224	3	0	0.3	0.150	0.296	ns	3	0	0.0	0.150	0.141	ns
Mean		3.3	0.184	0.184	2.9	0.9	0.00	0.189	0.185		2.4	0.4	0.06	0.179	0.182
PcHJM	P. cembra	5	0.390	0.468	4	1	0.1	0.381	0.481	ns	4	1	0.0	0.400	0.446	ns
RPS119	P. strobus	7	0.375	0.774	6	1	0.4	0.350	0.794	***	6	1	0.2	0.400	0.710	ns
RPS124	P. strobus	4	0.184	0.218	3	0	0.3	0.111	0.202	ns	4	1	0.0	0.250	0.229	ns
RPS127	P. strobus	9	0.275	0.804	9	3	0.5	0.238	0.814	***	6	0	0.3	0.316	0.737	***
P5	P. koraiensis	7	0.289	0.771	6	2	0.4	0.300	0.743	***	5	1	0.2	0.350	0.745	***
P29	P. koraiensis	4	0.450	0.363	3	1	0.0	0.450	0.366	ns	3	1	0.0	0.450	0.359	ns
P37	P. koraiensis	7	0.475	0.697	5	1	0.3	0.350	0.690	***	6	2	0.1	0.600	0.699	**
P38	P. koraiensis	11	0.353	0.470	8	5	0.2	0.300	0.428	ns	5	2	0.1	0.200	0.271	*
P45	P. koraiensis	15	0.563	0.893	13	3	0.2	0.571	0.892	*	12	2	0.2	0.579	0.892	ns
P52	P. koraiensis	5	0.512	0.647	4	1	0.2	0.381	0.602	ns	4	1	0.0	0.650	0.681	ns
P62	P. koraiensis	3	0.487	0.489	3	1	0.0	0.474	0.517	ns	2	0	0.0	0.500	0.455	ns
P63	P. koraiensis	8	0.375	0.714	8	2	0.4	0.350	0.739	***	6	0	0.2	0.400	0.666	**
PisATG0012	P. parviflora	8	0.400	0.659	6	2	0.1	0.500	0.648	ns	6	2	0.2	0.300	0.648	**
Mean		7.2	0.394	0.602	6.0	1.8	0.25	0.369	0.614		5.3	1.1	0.12	0.420	0.591

Source species for primer development, and genetic diversity for 10 microsatellite loci isolated for Pinus albicaulis Engelm. (Pinaceae) and 13 microsatellite loci transferred, based on 20 individuals from each population. (Henderson Mountain, Custer Gallatin National Forest, MT, and Mount Washburn, Yellowstone National Park, WY.)

N_a = total number of alleles; H_o = observed heterozygosity, total sample; H_e = expected heterozygosity, total sample; N_A = number of alleles; A_P = private alleles; A_N = null allele frequency; H_O = observed heterozygosity; H_E = expected heterozygosity; HWE = Hardy-Weinberg Equilibrium (ns = p > 0.05; * = p <0.05; ** = p < 0.01; *** = p < 0.001)