Fig 4. Competitive fitness studies in response to diphthamide defects, EF2 undersupply or both.

(A) Experimental setup. Equal amounts of wild-type (wt) and mutant (xxxΔ) cells were mixed and grown for 24 h (1 d) and successively passaged three more times for a total period of 96 h (4 d). After each passage, the relative amount (% of cells) of the two cell-types was determined. Growth experiments involving competition between wt and dph2Δ (B), eft2Δ (C) or dph2Δ eft2Δ (D) cells are shown.