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. 2018 Oct 18;13(10):e0205870. doi: 10.1371/journal.pone.0205870

Fig 7. Growth response to translation indicator drugs and vulnerability to programmed -1 frame-shifting of mutants lacking diphthamide and/or proper EF2 supply (dph2Δ, eft2Δ and dph2Δ eft2Δ).

Fig 7

(A) Serial cell dilutions of the indicated yeast stains were cultivated on media without any drugs (untreated) or supplemented with various doses of anisomycin (10, 20 μg/ml) or hygromycin (40, 80 μg/ml) at 30°C for 2–3 days. (B) Programmed -1 frame-shift assays based on a dual luciferase reporter system (scheme) carrying a viral -1 frame-shift site: 5’-GGGTTTA-3’ [62]. Luminescence based read-outs were statistically analyzed using two-tailed t-test. * (P ≤ 0.05); n.s. (P > 0.05).