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. 2018 Oct 8;14(10):e1007709. doi: 10.1371/journal.pgen.1007709

Fig 6. Purines mediate activation of the BrsRM LRS.

Fig 6

A) The brsRM-gusA reporter was spotted onto chemically defined medium agar plates ± adenine (A) or guanine (G) and grown for four days. The concentrations of the purines supplemented in the agar plates are listed above or below the respective images. In addition, each of the brsRM-inducing transposon insertion mutations was introduced into the brsRM-gusA reporter strain and spotted onto chemically defined medium agar plates containing either B) no purines (–AG), C) 0.132 mM guanine (+G), D) 0.15 mM adenine (+A), or E) 0.132 mM guanine + 0.15 mM adenine (+AG). Strains are listed from left to right as: WT (parent reporter strain), 1 (tilS mutant), 2 (rpoB mutant), 3 (SMU_2060/2061 IGR insertion), 4 (rgpD mutant), 5 (ssuE mutant), 6 (SMU_1406c mutant), 7 (prfC mutant), 8 (SMU_1193 mutant), 9 (mnmE mutant), 10 (SMU_1297 mutant), and 11 (comE mutant). The strains were incubated for two days before imaging.