Table 4. brsRM-inducing transposon mutations.
| Strain | Function | Tn Insertion | *brsRM+ | *ΔbrsRM |
|---|---|---|---|---|
| Parent | brsRM-gusA (all other LRS deleted) | – | – | – |
| tilS (SMU_13) | tRNA(Ile)-lysidine synthase | 2 | ++++ | – |
| rpoB (SMU_1990) | RNA Polymerase beta subunit | 1 | +++ | – |
| SMU_2060–2061 | IGR between SMU_2060–2061 ORFs | 1 | +++ | +++ |
| rgpD (SMU_828) | Polysaccharide ABC transporter subunit | 1 | ++ | ++ |
| ssuE (SMU_1089) | NADPH-dependent FMN reductase | 1 | ++ | – |
| SMU_1406c | NADPH-dependent FMN reductase | 3 | ++ | – |
| prfC (SMU_608) | Peptide chain release factor 3 | 4 | + | – |
| SMU_1193 | GntR Family transcription regulator | 1 | + | – |
| mnmE (SMU_1235) | tRNA modification GTPase | 3 | + | – |
| SMU_1297 | DHH Family phosphoesterase | 2 | + | – |
| comE (SMU_1917) | Sensor kinase | 1 | + | – |
*++++ indicates strongest GusA reporter activity on complex medium agar plates
*+++ indicates strong GusA reporter activity on complex medium agar plates
*++ indicates medium GusA reporter activity on complex medium agar plates
*+ indicates weak GusA reporter activity on complex medium agar plates