Figure 4. MAPLE performance at collecting virgin female flies for genetic crosses.

(A) Diagram of modules and procedure employed in MAPLE virgin-collecting. (B) Comparison of cumulative number of virgin females collected versus manual labor time required in MAPLE and conventional manual virgining procedures. Dotted lines indicate the maximum potential virgins that might have been collected in each approach. Thus the difference between the dotted and solid line indicates females that were ‘lost’ and not retained as virgins.

Figure 4—figure supplement 1. Outcome of a virgin-collecting experiment.

Representative illustration of the contents of the wells of a full FlyPlate after 3 days of virgin collecting. Outer circle color indicates the number of flies loaded. Inner circles indicate the sex of deposited flies. Stacked bars at right indicate the number of flies isolated by sex and virgin status.