Table 1.

List of reagents used to acutely perturb CME at synapses and their effects. Details of their effects on synaptic morphology and function, as well as control reagents, are described in the references.

STAGE OF CME AFFECTED	REAGENT	BIOCHEMICAL ACTIONS	SEQUENCE	REFS
CLATHRIN ASSEMBLY	AP2 peptide	Binds clathrin N-terminal domain and inhibits clathrin assembly in vitro and in vivo	QGDLLGDLLNLDLGPPVNVPQ	Morgan et al., 2000; Zhou et al., 2015;Fig. 3
	AP180 peptide	Inhibits clathrin assembly in vitro and in vivo	SGGATAWGDLLGEDSLAALSS	Morgan et al., 2000
	Pitstop 1	Inhibits ligand interactions with clathrin N-terminal domain	n.a.	von Kleist et al., 2011
CLATHRIN COAT MATURATION	Endophilin Antibody	Inhibits CME and vesicle recycling in vivo; build up of shallow CCPs	Antibody raised against a peptide corresponding to a.a. 255-274 of rat endophilin (YQPKPRMSLEFATGDGTQPN)	Ringstad et al., 1999
	Actin Toxins (Phalloidin, Bot C2; Lat B, Swinholide)	Inhibit actin dynamics in vivo	n.a.	Bourne et al., 2006; Shupliakov et al., 2002
	PIP kinase peptide	Inhibits actin polymerization in vivo	TDERSWVYSPLHYS	Morgan et al., 2004
CONSTRICTION & FISSION	Dynamin peptide	Inhibits dynamin-amphiphysin SH3 interaction	PPPQVPSRPNRAPPG	Shupliakov et al., 1997
	Amphiphysin SH3 domain	Binds dynamin in vitro and prevents recruitment to CCP	GST-tagged SH3 domain of human amphiphysin (a.a. 623-694)	Shupliakov et al., 1997
FISSION & UNCOATING	Synaptojanin peptide	Inhibits endophilin interactions with dynamin and synaptojanin in vitro	VAPPARPAPPQRPPPPSGA	Gad et al., 2000
	Auxilin ΔHPD mutant	Inhibits Hsc70-auxilin interaction and clathrin uncoating in vitro	Recombinant bovine auxilin truncation (a.a. 547-910) with mutated HPD motif (a.a. 874-876)	Morgan et al., 2001
	α-Synuclein	Binds Hsc70	Recombinant full-length human α-synuclein (a.a. 1-140)	Banks et al., 2015